Sarah VanOeveren PhotoSarah VanOeveren

Germline Clonal Analysis of the delorean Mutation in Drosophilia melanogaster

We study a gain-of-function mutation in Drosophila melanogaster, known as delorean, caused by a transposon insertion in an intron of the Protein kinase N (pkn) gene.  Preliminary evidence suggests that the delorean mutation is associated with a maternal-effect phenotype.  During oogenesis, maternal-effect genes are transcribed in the both the germline and somatic cells that communicate to produce a functional egg and support zygotic development. The germline-specific role of the delorean mutation was studied using the FLP-DFS (Flp recombinase target-dominant female sterile) technique to generate germline clones (GLC). Chromosomes were engineered to contain both the delorean mutation and DNA sequence (FRT sites) required for mitotic recombination upon induction of FLP recombinase. The products will include a cell that is homozygous for the delorean allele and another that is homozygous for the dominant female sterile mutation ovoD1.  This approach allows the pkn gene expressed in oogenesis to be studied in order to gain insight into the molecular mechanisms that determine oocyte developmental competence.

Faculty Mentor: Georgette Sass, Biology

Page last modified July 16, 2012