Kathryn Lauer ACF Abstract FY12

"The Effect of Room Temperature Storage of Patient Serum Specimens on Troponin T Levels"

2012 American Society for Clinical Laboratory Science-Michigan Annual Meeting and Exhibits

The purpose of this study is to determine the effect of room temperature storage of patient serum specimens on troponin T levels. A baseline level of troponin T will be measured and the specimens will be incubated at room temperature. Levels will be measured again two hours, four hours, and eight hours after the baseline measurement to observe the effect of room temperature incubation on troponin T levels. The Cobas e 602 electrochemiluminescence immunoassay module is manufactured by Roche and is part of the Cobas 8000 analyzer series. It represents the newest technology for measuring a variety of chemistry analytes, including Troponin T. The protocol for troponin T testing requires a blood sample collected in a lithium heparin tube that is separated via centrifugation. Once the specimens are centrifuged the serum is separated from the red blood cells by a gel separator and no further centrifugation is required. According to instrument protocol, the serum must be tested within two hours of the blood draw or the test results are invalid. If the sample will not be tested within two hours, the uncentrifuged specimen may be stored at four degrees Celsius for up to 24 hours and the troponin T levels will remain stable. Larger reference laboratories often perform Troponin T blood level testing for smaller laboratories. During transport, specimen integrity is lost if the specimen does not reach the testing laboratory within the two hour time limit. If the results of this study indicate there to be minimal to no change in Troponin T levels after the specimen is stored at room temperature for greater than two hours, a greater amount of time will be available to transfer the specimen to the testing laboratory and fewer specimens will be rejected for testing.

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