Benjamin Hake ACF Abstract FY12

"Changes in vascular reactivity of mesenteric arteries following hyperbaric oxygen treatment"

American Physiological Society Experimental Biology 2012

The objective of this study was to evaluate the changes in vascular
reactivity of mesenteric arteries resulting from hyperbaric oxygen
treatment. It was hypothesized that hyperbaric oxygen treatment
alters vascular reactivity in mesenteric arteries. Arteries from
porcine mesenteries were dissected and then subjected to a 2-hour
hyperbaric treatment in 100% oxygen, 100% nitrogen, or ambient
air (1.75 ATA). An additional control included vessels exposed to
ambient air at 1 ATA. Immediately following, arteries were
mounted in isolated organ baths and passively loaded with 7 grams
of tension. Following a 1-hour equilibration in Krebs-Henseleit
solution arteries were treated with potassium chloride (KCl; 15 –
60 mM) to assess viability. Subsequently, arteries were treated
with increasing concentrations of phenylephrine (10-7 – 10-4 M)
then sodium nitroprusside (10-7 – 10-4 M) to measure changes in
vascular reactivity. KCl-induced vasoconstriction was significantly
increased in response to hyperbaric oxygen treatment compared to
ambient air control (1 ATA). Treatment with hyperbaric oxygen
augmented vascular responses to phenylephrine and sodium
nitroprusside relative to nitrogen but not ambient air. The results
from these studies provide insight into the vascular effects of
hyperbaric oxygen treatment.


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