Associate Professor Biomedical Science Department 234 Padnos Hall Allendale, Michigan 49401 Phone: 616-331-3019
See available times here, or by appointment
BMS 202 - Human Anatomy and Physiology
BMS 202L - Laboratory in Human Anatomy and Physiology BMS 208 - Human Anatomy
BMS 290 - Human Physiology
BMS 291 - Laboratory in Human Physiology BMS 309 - Human Anatomy Cadaver Laboratory BMS 310 - Basic Pathophysiology BMS 365 - Exercise Physiology BMS 375 - Biology of Human Aging BMS 427 - Neuroanatomy BMS 428 - Neuroscience BMS 495 - Capstone
Ph.D. (1994) Wayne State University School of Medicine
M.S. (1991) Wayne State University School of Medicine B.S. (1987) Mercy College of Detroit
We are examining a molecule called GAP-43 which is a brain protein that is expressed in a wide variety of species including humans and has been shown to become biochemically altered in the process of learning and memory. Specifically, levels of phosphorylated forms of GAP-43 have been shown to increase following a controversial paradigm of learning and memory in several animals including rat, mouse and rabbit. We are interested to see if any differences in the profile of GAP-43 are associated with dementing illnesses that severely disrupt memory and learning. Since human brain tissue is difficult to obtain, we utilize brain tissue from a genetically altered mouse engineered to resemble Alzheimer's disease, a human neurodegenerative disorder characterized by profound cognitive impairment. Therefore, to test the hypothesis that the profile of phosphorylated isoforms of GAP-43 are changed in the brains of a mouse used to model Alzheimer's disease, GAP-43 will be examined by 1 and 2 dimensional SDS polyacrylamide gel electrophoresis. Isoforms of mouse brain GAP-43 will be detected by immunocytochemistry and silver staining and, further, quantified by computerized densitometry. Alterations in quantities of phosphorylated forms of GAP-43 might result from a pathological biochemical processes. Revealing molecular defects generates potential targets for the development of possibly more effective drugs to combat dementia.
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